OOActive (Artificial Oocyte Activation) with Calcium Ionophore
OOActive is Artificial Oocyte Activation (AOA) with calcium ionophore-an advanced IVF lab technique used after ICSI for selected patients who’ve faced failed or very low fertilisation. Sometimes, even when the sperm is injected correctly during ICSI, the egg doesn’t “switch on” (activate) the way it should. OOActive supports that activation step by using a calcium ionophore solution, which helps increase calcium inside the egg-similar to the natural signal the sperm usually triggers.
Why OOActive (AOA with Calcium Ionophore) Is Used
OOActive is considered only when your case strongly suggests an egg activation issue after ICSI. Professional guidance also recommends restricting AOA to very specific situations (not for everyone).
Failed or Very Low Fertilisation After ICSI
If fertilisation is failed or consistently low, AOA with calcium ionophore may help trigger activation so fertilisation can begin.
Repeated ICSI Cycles With 30% or No Fertilisation
International best-practice guidance suggests considering AOA when previous routine ICSI cycles resulted in 30% or no fertilisation (after proper review of egg/sperm/ICSI quality).
Suspected Egg Activation Deficiency
Some eggs don’t release the internal calcium signal needed to start fertilisation. AOA supports this calcium-based activation pathway.
Specific Male-Factor Scenarios
In some male-factor conditions (including rare cases like globozoospermia), the sperm may lack the factor needed to activate the egg, and AOA can be particularly helpful in this subgroup.
Unexplained Fertilisation Failure
If standard checks look normal but fertilisation still fails, OOActive may be discussed as a next-step lab strategy.
How OOActive (AOA with Calcium Ionophore) Works
OOActive is done immediately after ICSI inside the embryology lab:
Does OOActive Improve IVF Success?
OOActive can improve fertilisation rates in selected patients, especially those with prior low/failed fertilisation after ICSI.
OOActive Treatment & Support Options at India IVF
Advanced fertility treatments supported by experienced specialists, modern technology, and structured care throughout your treatment journey.
Ready to Take the Next Step?
If you’ve had failed fertilisation after ICSI or repeated low fertilisation, speak with the fertility team at India IVF to understand whether OOActive (AOA with calcium ionophore) fits your case.
OOActive (Artificial Oocyte Activation with Calcium Ionophore)
Understanding your options is the first step toward hope. Here are some of the most common questions our fertility experts answer every day.